用途:用于培养链球菌，肺炎链球菌、脑膜炎球菌等苛养菌。
 成分*   含量 g/L
 牛脑浸粉7.7g
 牛心浸粉 9.8g
 月示蛋白胨 10.0
 葡萄糖  2.0
 氯化钠  5.0
 磷酸二钠 2.5
pH 7.4±0.2
Bacto™ Brain Heart Infusion Approximate Formula* Per Liter
Calf Brains, Infusion from 200 g  7.7 g
Beef Heart, Infusion from 250 g  9.8 g
Proteose Peptone  10.0 g Dextrose 2.0  g
Sodium Chloride 5.0  g
Disodium Phosphate 2.5 g
可根据操作标准自行调整配方
制备方法：
溶解37g培养基至1L蒸馏水中，混匀分装至最终容器中，121℃高压灭菌15分钟。
Directions：Suspend 37g of the powder in 1 L of purified water. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Autoclave at 121°C for 15 minutes. Test samples of the finished product for performance using stable, typical control cultures.
 
Bacto™ Brain Heart Infusion Dehydrated Appearance: Light tan, free-flowing, homogeneous. Solution: 3.7% solution, soluble in purified water upon boiling. Solution is light to medium amber, clear. Prepared Appearance: Light to medium amber, clear. Reaction of 3.7% Solution at 25°C: pH 7.4 ± 0.2
BHI肉汤是一种营养丰富的缓冲培养基，含有大脑和心脏组织以及胃蛋白酶，以提供蛋白质和其他必要的营养物质来支持挑剔和不挑剔的微生物的生长。在含有6.5% 氯化钠的配方中，盐作为一种差异和/或选择性介质，干扰不耐盐生物的膜通透性和渗透动力学平衡。滤丝富集法(羊血消化法)被纳入一种管状配方，用于培养流感嗜血桿菌等较为挑剔的微生物。