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产品信息
| 名称 | Daoy (人髓母细胞瘤细胞) (STR鉴定正确) |
| 别称 | DAOY; D324 Med; D-324 Med; D324 MED; D-324MED; D324 |
| 背景描述 | The Daoy cell line was established in 1985 by P. F Jacobsen of the Royal Perth Hospital in Western Australia. The line was derived from biopsy material taken from a tumor in the posterior fossa of a 4 year old boy. |
| 种属 | 人 |
| 年龄（性别） | 男；4岁 |
| 组织来源 | 结缔组织增生性小脑髓母细胞 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 神经母细胞瘤细胞 |
| 生物安全等级 | 1 |
| 生长培养基 | MEM（含NEAA）＋10% FBS＋1%P/S |
| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2~3次/周 |
| 倍增时间 | 33.6 hours, at 48th passage (PubMed=2993532); 34 hours (ATCC) |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5%温度：37℃ |
| 致瘤性 | Yes, in nude mice(The cells form serially transplantable intercranial and subcutaneous tumors.) |
| 保藏机构 | ATCC; HTB-186 BCRJ; 0325 |
STR位点信息
| Amelogenin | X |
| CSF1PO | 11 |
| D2S1338 | 16,17 |
| D3S1358 | 15 |
| D5S818 | 11,13 |
| D7S820 | 8,10 |
| D8S1179 | 13,15 |
| D13S317 | 13,14 |
| D16S539 | 10 |
| D18S51 | 12 |
| D19S433 | 14.2 |
| D21S11 | 29,31.2 |
| FGA | 23 |
| PentaD | 10,13 |
| PentaE | 7,11 |
| TH01 | 9 |
| TPOX | 8,10 |
| vWA | 14,20 |
| D6S1043 | 12 |
| D12S391 | 20 |
| D2S441 | 10,11 |
