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产品信息
| 名称 | MIA PaCa-2 (人胰腺癌细胞) (STR鉴定正确) |
| 别称 | MIA-PaCa-2; MIA-PACA-2; MIA-Pa-Ca-2; MIA Paca2; MIA PaCa2; MiaPaCa-2; MIAPACA-2; MiaPaca.2; MiaPaCa2; Miapaca2; MIAPaCa2; MIAPACA2; Mia PACA 2; MIAPaCa-2; PaCa2 |
| 背景描述 | The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. |
| 种属 | 人 |
| 年龄（性别） | 男；65岁 |
| 生长特性 | 半贴半悬 |
| 细胞形态 | 上皮样贴壁细胞，伴有圆形漂浮细胞 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 胰腺癌细胞 |
| 生物安全等级 | 1 |
| 生长培养基 | DMEM＋10%FBS＋2.5%HS＋1%P/S |
| 推荐传代比例 | 1:3-1:8 |
| 推荐换液频率 | 2~3次/周 |
| 倍增时间 | 26 hours (PubMed=25984343); 25.7 +- 4.3 hours (PubMed=27067801); 40 hours, 18 hours, in serum-free medium (PubMed=23386380); ~40 hours (ATCC); ~30-40 hours (DSMZ) |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5%温度：37℃ |
| 基因表达情况 | human colony stimulating factor, subclass I (CSF-I); plasminogen activator |
| 保藏机构 | ATCC; CRL-1420DSMZ; ACC-733ECACC; 85062806JCRB; JCRB0070 |
STR位点信息
| Amelogenin | X |
| CSF1PO | 10 |
| D2S1338 | 25 |
| D3S1358 | 16 |
| D5S818 | 12,13 |
| D7S820 | 12 |
| D8S1179 | 16 |
| D13S317 | 12,13 |
| D16S539 | 10,13 |
| D18S51 | 12 |
| D19S433 | 15 |
| D21S11 | 29,31.2 |
| FGA | 22 |
| PentaD | 12,16 |
| PentaE | 13,18 |
| TH01 | 9,10 |
| TPOX | 9 |
| vWA | 15 |
| D6S1043 | 12 |
| D12S391 | 19 |
| D2S441 | 14 |
