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| 名称 | KYSE-150 (人食管鳞癌细胞) (STR鉴定正确) |
| 别称 | KYSE 150; KYSE150; Kyse150; KY150 |
| 背景描述 | Established from the poorly differentiated esophageal squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old Japanese woman after receiving radiotherapy (tumor was invading contiguous structures); described as carrying amplified oncogenes, c-erb-B (8x) and cyclin D1 (4x) and producing tumors in nude mice. |
| 种属 | 人 |
| 年龄（性别） | 女；49岁 |
| 组织来源 | 食管 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 食管癌细胞 |
| 生物安全等级 | 1 |
| 生长培养基 | RPMI-1640＋Ham's F-12＋10% FBS＋1%P/S |
| 推荐传代比例 | 1:4-1:8 |
| 推荐换液频率 | 2~3次/周 |
| 倍增时间 | 13.7 hours (PubMed=1728357); 23 hours (PubMed=25984343); <25 hours, less when serum amount is increased (DSMZ) |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5%温度：37℃ |
| 致瘤性 | Yes, in nude mice |
| 保藏机构 | DSMZ; ACC-375 |
STR位点信息
| Amelogenin | X |
| CSF1PO | 12,13 |
| D2S1338 | 25 |
| D3S1358 | 15,16 |
| D5S818 | 12,13 |
| D7S820 | 10,11 |
| D8S1179 | 10,15 |
| D13S317 | 8,11 |
| D16S539 | 9,11 |
| D18S51 | 14 |
| D19S433 | 15,15.2 |
| D21S11 | 30,31 |
| FGA | 21,24 |
| PentaD | 10 |
| PentaE | 12,18 |
| TH01 | 7,9 |
| TPOX | 8 |
| vWA | 16,17 |
| D6S1043 | 18,20 |
| D12S391 | 19,22 |
| D2S441 | 10,11 |
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