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| 名称 | SU-DHL-4 (人B淋巴瘤细胞) (STR鉴定正确) |
| 别称 | SUDHL4; Sudhl4; SUDHL-4; Sudhl-4; SuDHL 4; SUD-4; SUD4; SU4; Stanford University-Diffuse Histiocytic Lymphoma-4; DHL-4; DHL4 |
| 背景描述 | Established from the peritoneal effusion of a 38-year-old man with B-NHL (diffuse large cell, cleaved cell type; originally described as "diffuse histiocytic lymphoma") in 1975; cell line carries EZH2 Y641S mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| 种属 | 人类 |
| 年龄（性别） | 男性，38岁 |
| 组织来源 | 腹腔积液 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 淋巴母细胞样 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 淋巴瘤细胞 |
| 生长培养基 | RPMI-1640＋10% FBS＋1% P/S |
| 推荐传代比例 | 5×10^5-1×10^6cells/mL |
| 推荐换液频率 | 2~3次/周 |
| 倍增时间 | ~40小时 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5%温度：37℃ |
| 基因表达情况 | IgG+; Kappa+; IgM-; IgA-; IgD-; Lambda-; This cell line has relatively high expression levels of Bax; Bak; AIF; high caspase-9 activity. |
| 注意事项 | 该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。 |
| 保藏机构 | ATCC; CRL-2957 DSMZ; ACC-495 |
STR位点信息
| Amelogenin | X,Y |
| CSF1PO | 12 |
| D2S1338 | 17,19 |
| D3S1358 | 15,16 |
| D5S818 | 11,12 |
| D7S820 | 8,11 |
| D8S1179 | 13 |
| D13S317 | 12 |
| D16S539 | 11,13 |
| D18S51 | 14,16 |
| D19S433 | 14,15.2 |
| D21S11 | 29,31 |
| FGA | 19,21 |
| PentaD | 9,14 |
| PentaE | 7,12 |
| TH01 | 6,9.3 |
| TPOX | 9,11 |
| vWA | 18,19 |
| D6S1043 | 11 |
| D12S391 | 18,20 |
| D2S441 | 11,14 |
