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产品信息
| 名称 | NCI-H146 [H146] (人小细胞肺癌细胞) (STR鉴定正确) |
| 别称 | H146; H-146; NCIH146 |
| 背景描述 | Derived from the pleural fluid of a patient with small cell lung cancer; the cells express elevated levels of the 4 biochemical markers characteristic of SCLC: neuron-specific enolase, the brain isoenzyme of creatine kinase, L-dopa decarboxylase and bombesin-like immunoreactivity; the cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein; the cells express relatively high amounts of c-myc mRNA, but no amplification of the gene is detected. |
| 种属 | 人类 |
| 年龄（性别） | 男性，59岁 |
| 组织来源 | 肺 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 淋巴母细胞样 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肺癌细胞 |
| 生长培养基 | RPMI-1640＋10% FBS＋1% P/S |
| 推荐传代比例 | 1:2-1:3 |
| 推荐换液频率 | 2~3次/周 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5%温度：37℃ |
| 基因表达情况 | vasopressin negative; oxytocin negative; gastrin releasing peptide negative; vimentin positive by immunostaining; keratin positive by immunostaining; neurofilament triplet protein negative; c-myb mRNA; v-fms mRNA; Ha-ras mRNA; Ki-ras mRNA; N-ras mRNA; c-raf 1 mRNA; myc+; myb+; raf+; ras+; fms+; fes - |
| 注意事项 | 该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。 |
| 保藏机构 | ATCC; HTB-173 |
STR位点信息
| Amelogenin | X |
| CSF1PO | 11 |
| D2S1338 | 19 |
| D3S1358 | 11 |
| D5S818 | 12 |
| D7S820 | 9,10 |
| D8S1179 | 12,14 |
| D13S317 | 12 |
| D16S539 | 11 |
| D18S51 | 15,17 |
| D19S433 | 13,14 |
| D21S11 | 30 |
| FGA | 23 |
| PentaD | 13 |
| PentaE | 12 |
| TH01 | 6,9.3 |
| TPOX | 8,11 |
| vWA | 14,16 |
| D6S1043 | 11,19 |
| D12S391 | 18.3,25 |
| D2S441 | 10,14 |
