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产品信息
| 名称 | KYSE-30 (人食管鳞癌细胞) (STR鉴定正确) |
| 别称 | KYSE 30; KYSE30; KYSE0030 |
| 背景描述 | Derived from well differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 64-year-old Japanese man prior to treatment; cell line Established from tumor cells heterotransplanted into athymic mice; described in the literature to be heterotransplantable in athymic mice and to carry p53 mutation and amplification of cERB B, MYC and CYCLIN D1 |
| 种属 | 人 |
| 年龄（性别） | 女；64岁 |
| 组织来源 | 食管鳞状上皮 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样，带有长的伪足 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 食管癌细胞 |
| 生物安全等级 | 1 |
| 生长培养基 | RPMI-1640＋Ham's F-12＋2mM L-Glutamine＋10% FBS＋1% P/S |
| 推荐传代比例 | 1:3-1:5 |
| 推荐换液频率 | 2~3次/周 |
| 倍增时间 | ~20-30 hours |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5%温度：37℃ |
| 保藏机构 | DSMZ; ACC-351 ECACC; 94072011 JCRB; JCRB0188 |
STR位点信息
| Amelogenin | X |
| CSF1PO | 10 |
| D2S1338 | 23 |
| D3S1358 | 15,16 |
| D5S818 | 11 |
| D7S820 | 11,11.3 |
| D8S1179 | 12,15 |
| D13S317 | 9 |
| D16S539 | 10,12 |
| D18S51 | 14 |
| D19S433 | 14.2,15.2 |
| D21S11 | 28 |
| FGA | 24 |
| PentaD | 12 |
| PentaE | 13 |
| TH01 | 9 |
| TPOX | 9 |
| vWA | 16,18,19 |
| D6S1043 | 11,20 |
| D12S391 | 17,19 |
| D2S441 | 11 |
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