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| 名称 | MT-4 (人T细胞白血病细胞) (STR鉴定正确)（仅提供冻存管） |
| 背景描述 | Derived from a 50 year old Japanese male with generalised lymphadenopathy and heptosplenomegaly (Adult T-cell Leukaemia) by co-culture of his peripheral leukocytes with male umbilical cord lymphocytes. The cells carry HTLV-1 and support the growth of HIV. The cells should be handled under laboratory containment level 3 conditions. |
| 种属 | 人 |
| 组织来源 | 淋巴细胞 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 淋巴细胞样 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 淋巴瘤细胞 |
| 生长培养基 | RPMI-1640＋10% FBS＋1% P/S |
| 推荐传代比例 | 3×10^5-5×10^5cells/mL |
| 推荐换液频率 | 2~3次/周 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% |
| 注意事项 | 该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。 |
STR位点信息
| Amelogenin | X,Y |
| CSF1PO | 11,12 |
| D2S1338 | 18,23 |
| D3S1358 | 17 |
| D5S818 | 10,11 |
| D7S820 | 8,10 |
| D8S1179 | 10,15 |
| D13S317 | 12 |
| D16S539 | 9,12 |
| D18S51 | 13 |
| D19S433 | 14.2,15.2 |
| D21S11 | 28,30.3 |
| FGA | 23 |
| PentaD | 10,13 |
| PentaE | 5,15 |
| TH01 | 7 |
| TPOX | 11 |
| vWA | 17,18 |
| D6S1043 | 12 |
| D12S391 | 18,21 |
| D2S441 | 10 |
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