PE/Dazzle™ 594 anti-mouse CD133 Antibody

Description                        

CD133 (also known as prominin-1 and AC133) is a 120 kD pentaspan glycoprotein with 5 transmembrane domains which localize to cellular protrusions. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to hematopoietic stem cells, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. It also has been detected at low levels in the kidney, pancreas, placenta, and fetal liver tissue. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.

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Pricing & Availability                

• Clone

• 315-2C11 (See other available formats)

• Regulatory Status

• RUO

• Other Names

• Prominin-1, AC133

• Isotype

• Rat IgG2a, λ

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Description                        

CD133 (also known as prominin-1 and AC133) is a 120 kD pentaspan glycoprotein with 5 transmembrane domains which localize to cellular protrusions. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to hematopoietic stem cells, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. It also has been detected at low levels in the kidney, pancreas, placenta, and fetal liver tissue. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.

Product Details                    

Product Details            

• Isotype Control

• PE/Dazzle™ 594 Rat IgG2a, κ Isotype Ctrl

• Verified Reactivity

• Mouse

• Antibody Type

• Monoclonal

• Host Species

• Rat

• Immunogen

• CD133-expressing-GFP-RBL2H3 plus CFA as adjuvant.

• Formulation

• Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

• Preparation

• The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.

• Concentration

• 0.2 mg/ml

• Storage & Handling

• The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

• Application

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  FC - Quality tested

• Recommended Usage

• Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
  
  * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

• Excitation Laser

• Blue Laser (488 nm)
  Green Laser (532 nm)/Yellow-Green Laser (561 nm)
  

• Application References
  
  (PubMed link indicates BioLegend citation)

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1. Zhang Y, et al. 2012. Diabetes. 61:2114. PubMed

• RRID

• AB_2566009                                (BioLegend Cat. No. 141211)                                
  AB_2566010                                (BioLegend Cat. No. 141212)